2018/10/27 Saturday H3C0、 K-ocH OH OH O 200T180T160T10T 6
2018/10/27 Saturday 6 O O OCH3 OH H3CO OH 191 O O OCH3 OH H3CO OH 192
2018/10/27 Saturday H-H COSY H.C 193 60 4036 HMQC OCH: 60 66 7
2018/10/27 Saturday 7 O O OCH3 OH H3CO OH 1H-1H COSY 193 HMQC O O OCH3 OH H3CO OH 194
2018/10/27 Saturday HMBC OCH OH 195 HMBC H:C Me 75 60 55 50 45 8
2018/10/27 Saturday 8 HMBC O O OCH3 OH H3CO OH 195 HMBC O O OCH3 OH H3CO MeO OMe OH 196
2018/10/27 Saturday HMBC OCH, 197 HMBC 198 9
2018/10/27 Saturday 9 HMBC OCH3 OH O H3CO OCH3 OH 197 H2C CH3 OH H O O H 4 H 3 H HO H 2 CH3 HMBC 198
2018/10/27 Saturday 波谱法特点 ◆()样品用量少，一般来说2-3mg即可 （最低可少到<1mg)； ·(②)除质谱外，其它方法无样品消耗，可回收再 使用； ◆(3)省时、简便 ◆（④）配合元素分析（或高分辨质谱)，可以准确 地确定化合物的结构 19g Ciguatoxin CTX 如人·有萧有-含鼠明 1.1 mg from 4000 Kg of moray eels A C55 fatty acid coiled into one terminal spiro and twelve contiguous transfused ether rings ranging in size from oxolane to oxonane.The remaining structural features were unremarkable-five olefins,five methyls,and six hydroxyls,two as a terminal vicinal diol. LD00.254g/kg)200 10
2018/10/27 Saturday 10 波谱法特点 (1) 样品用量少，一般来说2-3 mg即可 （最低可少到<1 mg）； (2) 除质谱外，其它方法无样品消耗，可回收再 使用； (3) 省时、简便 (4) 配合元素分析（或高分辨质谱），可以准确 地确定化合物的结构 199 Ciguatoxin CTX A C55 fatty acid coiled into one terminal spiro and twelve contiguous transfused ether rings ranging in size from oxolane to oxonane. The remaining structural features were unremarkable-five olefins, five methyls, and six hydroxyls, two as a terminal vicinal diol. O O O O O O O O O O O O O H H H H H HO H H H H OH H OH H H H H H H H H H H OH HO OH (LD50 0.25-4 μg/kg) 1.1 mg from 4000 Kg of moray eels 200