◼ 生产上常用孢子培养基: 麸皮、大米、小米培养基;由葡萄糖、蛋白 胨、牛肉膏和食盐等配制的琼脂斜面培养基。 ▪ 大米和小米常用作霉菌孢子培养基,因它们 含氮少、疏松、表面积大,是较好的孢子培 养基。水分控制在21%-25%
◼ 生产上常用孢子培养基: 麸皮、大米、小米培养基;由葡萄糖、蛋白 胨、牛肉膏和食盐等配制的琼脂斜面培养基。 ▪ 大米和小米常用作霉菌孢子培养基,因它们 含氮少、疏松、表面积大,是较好的孢子培 养基。水分控制在21%-25%
◼ 种子培养基 inoculum media: 种子培养基是供孢子发芽、生长和大量繁 殖菌丝体,并使菌丝体长得粗壮,成为活 力强的‘种子’ 。 ◼ Inoculum media are usually less nutritious than are production media, and they usually contain a considerably lower level of the main nutritive carbon source
◼ 种子培养基 inoculum media: 种子培养基是供孢子发芽、生长和大量繁 殖菌丝体,并使菌丝体长得粗壮,成为活 力强的‘种子’ 。 ◼ Inoculum media are usually less nutritious than are production media, and they usually contain a considerably lower level of the main nutritive carbon source
◼ 一般种子培养基常包括有机氮源和无机氮源 ◼ 最后一级的种子培养基的成分最好接近发酵培 养基。 ◼ 发酵培养基 production media 发酵培养基是供菌体生长、繁殖和合成产物之 用。 要求:既要使种子接种后能迅速生长,达到一 定的菌丝浓度,又要使长好的菌体迅速合成所 需产物
◼ 一般种子培养基常包括有机氮源和无机氮源 ◼ 最后一级的种子培养基的成分最好接近发酵培 养基。 ◼ 发酵培养基 production media 发酵培养基是供菌体生长、繁殖和合成产物之 用。 要求:既要使种子接种后能迅速生长,达到一 定的菌丝浓度,又要使长好的菌体迅速合成所 需产物
◼ 配制要求:其组成除有菌体生长所必需 的元素和化合物外,还需有合成产物所 需的特定元素、前体和促进剂等。 ◼ In addition to the carbon and nitrogen compounds, a medium contains inorganic salts, vitamins, growth factors, precursors of fermentation products
◼ 配制要求:其组成除有菌体生长所必需 的元素和化合物外,还需有合成产物所 需的特定元素、前体和促进剂等。 ◼ In addition to the carbon and nitrogen compounds, a medium contains inorganic salts, vitamins, growth factors, precursors of fermentation products
◼ Microbial growth under industrial fermentation conditions usually utilizes a “luxury metabolism” of the organisms. Thus, good yields of fermentation products occur only if relatively large amounts of carbon, and possibly nitrogen, are channeled through the metabolic pathways of the microorganism
◼ Microbial growth under industrial fermentation conditions usually utilizes a “luxury metabolism” of the organisms. Thus, good yields of fermentation products occur only if relatively large amounts of carbon, and possibly nitrogen, are channeled through the metabolic pathways of the microorganism