4. The principle of chromatography →--1------1--1--4--- Question 1: How chemically similar compounds can be separated in a chromatographic column? P2-3 sample mobile phase A+BEa B B ll B A目B呵
4. The principle of chromatography Question 1 : How chemically similar compounds can be separated in a chromatographic column? P 2-3 1 2 3 4 5 t t t t t sample mobile phase A+B B B B B A A A
5. Chromatogram and some terminology Base line Total retention time t-tM Dead time 20 Zone broadening 607h
5. Chromatogram and some terminology Base line Total retention time Dead time Zone broadening
用来衡量色谱峰宽度的参数,有三种表示方法: (1) Standard deviation (ii) The half-band width Y IM (111) The base-line width 0.607h 2
用来衡量色谱峰宽度的参数，有三种表示方法： (ⅰ) Standard deviation (ⅱ) The half-band width (ⅲ) The base-line width
Section 2 Chromatographic theory 1. Thermal dynamic equilibrium 2. Plate theory 3. Rate theory 4. The resolution
Section 2 Chromatographic theory 1. Thermal dynamic equilibrium 2. Plate theory 3. Rate theory 4. The resolution
-1--4--4-----4--1--4--1 色谱理论需要解决的问题: (1)色谱分离过程的热力学和动力学问题。 (2)影响分离及柱效的因素与提高柱效的途径。 (3)柱效与分离度的评价指标及其关系
色谱理论需要解决的问题： (1)色谱分离过程的热力学和动力学问题。 (2)影响分离及柱效的因素与提高柱效的途径。 (3)柱效与分离度的评价指标及其关系