H Amino acid crck预言的 OoC-C-NH 分子转换器 R (adaptor) Amino acid binding site Adaptor UAU GA CUA GUC GG mRnA Nucleotide triplet coding for an amino acid
Crick预言的 分子转换器 (adaptor)
DNA recombination experiments that proved the genetic codons as triplets mRNA 5-G U AG C CUA CIG G AU---3 (+) Insertion G UAG C CUCAC G GA U Deletion G C UAC GG A U Insertion and GUAAGCCA CIG GAU deletion Reading frame restored
DNA recombination experiments that proved the genetic codons as triplets
Mutation experiment tells Triplets do not overlap, but there are three reading frames Nonoverlapping A U AC G AGU C code 2 3 Overlapping A UACGAGUC code 2 × 3 Reading frame 1 5-U UCUC GG A CC U GGA GA U UC A CA G U---3 Reading frame 2 UJUC C CUG GA C AG U Reading frame - UC UCG ACC UGGAG A UUC AC A GU---
Mutation experiment tells: Triplets do not overlap, but there are three reading frames
Pre-requirement for elucidation of the Genetic code 要有确定的已知序列的mRNA 要有同位素标记的氨基酸
Pre-requirement for elucidation of the Genetic code • 要有确定的已知序列的mRNA • 要有同位素标记的氨基酸
19554F, Marianne Grunberg Manago和 Severo ochoa从细菌中 发现了一个酶 polynucleotide phosphorylase(PP,催化以下反应 (NMP)+ NDP (NMP)n+1+ Pi 这是第一个发现的核酸聚合酶,为模 板非依赖性,在细菌中主要起降解 RNA的作用,但可用来随机合成RNA多 聚物
• 1955年，Marianne GrunbergManago 和 Severo Ochoa从细菌中 发现了一个酶，polynucleotide phosphorylase (PP), 催化以下反应： (NMP)n + NDP (NMP)n+1 + Pi 这是第一个发现的核酸聚合酶，为模 板非依赖性，在细菌中主要起降解 RNA的作用, 但可用来随机合成RNA多 聚物