Confocal Microscopy http://v.youku.com/v_show/id_XMjMyNzE3NjQ0.html
Confocal Microscopy http://v.youku.com/v_show/id_XMjMyNzE3NjQ0.html
Optical Sectioning and 3D reconstruction V
Optical Sectioning and 3D reconstruction x y z
Optical Sectioning and 3D reconstruction 口
Optical Sectioning and 3D reconstruction x y z
Optical Sectioning and 3D reconstruction 0 一·
Optical Sectioning and 3D reconstruction x y z =
Fluorescence Illumination of a single point Camera Tube lens Excitation light Emission light Objective lens Sample Problem-fluorescence is emitted along entire illuminated cone,not just at focus
Fluorescence Illumination of a single point Sample Objective lens Excitation light Tube lens Emission light Problem – fluorescence is emitted along entire illuminated cone, not just at focus Camera