防病 防止病害 生物农药的功用 杀虫 防止虫害 除草 防止草害 提高自然 产物的生物 农药活性 生物技术
防病 防止病害 生物农药的功用 杀虫 防止虫害 除草 防止草害 提高自然 产物的生物 农药活性 生物技术
1 An overview of DNA cloning Some conception need to know Some useful technology important to gene manipulation Enzymes for DNA cloning Vectors
1 An overview of DNA cloning Some conception need to know Some useful technology important to gene manipulation Enzymes for DNA cloning Vectors
Conception DNA cloning is to place a relatively short fragment of a genome, which might contain the gene or other sequence of interest, in an autonomously replicating piece of DNA, known as a vector, forming recombinant DNA, which can be replicated independently of the original genome, and normally in other host species altogether. Propagation of the host organism containing the recombinant DNA forms a set of genetically identical organism, or a clone. This process is called DNA cloning
Conception DNA cloning is to place a relatively short fragment of a genome, which might contain the gene or other sequence of interest, in an autonomously replicating piece of DNA, known as a vector, forming recombinant DNA, which can be replicated independently of the original genome, and normally in other host species altogether. Propagation of the host organism containing the recombinant DNA forms a set of genetically identical organism, or a clone. This process is called DNA cloning
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ØHost organism/cell: where the plasmids get multiplied and propagated faithfully, which is crucial for DNA cloning. ØHosts for DNA cloning vector Prokaryotic host : E. coli ( most cases) Eukaryotic host : Yeast (Saccharomyces cerevisiae), large fragments of human genome
ØHost organism/cell: where the plasmids get multiplied and propagated faithfully, which is crucial for DNA cloning. ØHosts for DNA cloning vector Prokaryotic host : E. coli ( most cases) Eukaryotic host : Yeast (Saccharomyces cerevisiae), large fragments of human genome