foreign dna bacterialcell restriction endonuclease 重组DNA技术流程简图
重组DNA技术流程简图 vector restriction endonuclease foreign DNA bacterial cell
foreign dna bacterialcell vector restriction endonuclease ligase ◎ 重组DNA技术流程简图 host
重组DNA技术流程简图 ligase vector restriction endonuclease foreign DNA bacterial cell host
foreign dna bacterialcell restriction endonuclease ligase ◎ 重组DNA技术流程简图 host
重组DNA技术流程简图 ligase vector restriction endonuclease foreign DNA bacterial cell host
狠制性内切核酸酶 限制酶:能够识别DNA链的特定部位并能 进行切割 冷 Type I 冷Type 冷Type 平末端( Blunt end)&粘性末端( Sticky end)
限制性内切核酸酶 限制酶：能够识别DNA链的特定部位并能 进行切割。 ❖ TypeⅠ ❖ TypeⅡ ❖ TypeⅢ 平末端(Blunt end) & 粘性末端(Sticky end)
狠制性内切核酸酶 HaeⅢ 5GGCC3′ 5GG|[CC_3′ 3CCGG5′" 3CC|[GG_5′ 5■GATC3′ 5■ GATC3′ Mbo I 3′CTAG口5′ 3CTAG口5′ 5 GGATCC]3′ Bamh I 5 GGATCC]3′ 3′ I CCT AGG5 3′ CCT AG[G5 5 CTGC AG 3 5| CTGCA C3′ Pst I 3 GACGTC5′ 3 GACGTc15″
限制性内切核酸酶 HaeⅢ 5’ 3’ 3’ 5’ G G C C C C G G 5’ 3’ 3’ 5’ G G C C C C G G MboⅠ 5’ 3’ 3’ 5’ G A T C C T A G 5’ 3’ 3’ 5’ G A T C C T A G BamHⅠ 5’ 3’ 3’ 5’ G G A T C C C C T A G G 5’ 3’ 3’ 5’ G C C T A G G A T C C G PstⅠ 5’ 3’ 3’ 5’ C T G C A G G A C G T C 5’ 3’ 3’ 5’ C T G C A G G A C G T C