材料与能源学院 School of Materials and Energy 《材料分子结构分析》 第七章核磁共振谱 Nuclear Magnetic Resonance Spectroscopy, NMR wvalp on moy 1aBiofianau 刘钰 电子科技大学 2021年2022年春季
第七章 核磁共振谱 刘 钰 电子科技大学 2021年-2022年 春季 《材料分子结构分析》 Nuclear Magnetic Resonance Spectroscopy， NMR
never forget how to dream 目录 ■7.1核磁共振概述 ■7.2核磁共振谱的基本原理 ■7.3核磁共振谱波谱仪 ■7.4样品的制备和测试 ■7.5核磁共振氢谱 ■7.6核磁共振氢谱解析 ■7.7核磁共振氢谱的应用 ■7.8核磁共振碳谱 ■7.9核磁共振碳谱的解析及应用 2
2 目录 ◼ 7.1 核磁共振概述 ◼ 7.2 核磁共振谱的基本原理 ◼ 7.3 核磁共振谱波谱仪 ◼ 7.4 样品的制备和测试 ◼ 7.5 核磁共振氢谱 ◼ 7.6 核磁共振氢谱解析 ◼ 7.7 核磁共振氢谱的应用 ◼ 7.8 核磁共振碳谱 ◼ 7.9 核磁共振碳谱的解析及应用
7.1核磁共振概述 never for fream 核磁共振的发展 1938 Discovery of nuclear magnetic resonance (NMR) 1946 Principle of solid state NMR(Bloch, Purcell) 1950 Resonance frequency depends on chemical environment(Proctor,Yu) 1953 Overhauser effect 1956 First NMR spectra of protein (Ribonuclease) 1965 Fourier Transform spectroscopy(Ernst) 3
3 核磁共振的发展 1946 Principle of solid state NMR (Bloch, Purcell) 1950 Resonance frequency depends on chemical environment (Proctor, Yu) 1953 Overhauser effect 1956 First NMR spectra of protein (Ribonuclease) 1965 Fourier Transform spectroscopy (Ernst) 1938 Discovery of nuclear magnetic resonance （NMR） 7.1 核磁共振概述
71核磁共振概述 neger forget 1973 Imaging tomography(Mansfieidj Sc2 TSEMM SL8 1985 First protein structure(bovine pancreatic trypsin inhibitor)in solution (Wuthrich) Figure 27.NMR structure of BPTI represented by a bundle of 20 con- formers superim- posed for best fit of the polypeptide backbone.The polypeptide back- bone is green,core side-chains are blue, and solvent-accessi- ble surface side- chains are red. 4
4 1985 First protein structure (bovine pancreatic trypsin inhibitor) in solution (Wüthrich) 1973 Imaging tomography (Mansfield) 7.1 核磁共振概述
never forget 7.1核磁共振概述 how to aream By now:More than 150 protein structures (M<60 000) Figure 28.Molecular model of hydrated BPTI in H.O solu- tion.The drawing shows an all-heavy- atom-presentation of one of the con- formers in Figure 27 (yellow)covered BPTI with a layer of hy- dration water mole- cules (dotted blue spheres). 40s Bound water 50s >103 s-1 (>15s) >1048 1700s- Protein dynamics 300s1 5
5 By now: More than 150 protein structures (M < 60 000) BPTI Bound water Protein dynamics 7.1 核磁共振概述