10.7 Reaction rate and rate equation 10.7.2 Continuous flow method doi:10.1006/mbi2000.4124availableonlineathttp:/www.idealibrary.comonIdEalj.mOlBial.(2000)303,773-795 JMB 几 Multiple-step Kinetic Mechanism of DNA-independent ATP Binding and Hydrolysis by Escherichia coll Replicative Helicase DnaB Protein: Quantitative Analysis Using the Rapid Quench-flow Method Surendran Rajendran, Maria J. Jezewska and Wlodzimierz Bujalowski Difficulty:(1)a large amount of solution is required;(2) steady and exact motion ./ of the detector. 0.05 0.1 0.15 Time(
Difficulty: (1) a large amount of solution is required; (2) steady and exact motion of the detector. 10.7 Reaction rate and rate equation 10.7.2 Continuous flow method
10.7 Reaction rate and rate equation 10.7.3 Stopped-Flow Measurements in real time Fluorescence not requiring large amounts of reagents(as Optical.i. Absorbance Path little as 1 cm3 may be sufficient) the time resolution is a little faster then for Mixer Stopping Syringe continuous flo Fixed probe strategy The usual dead time of a stopped flow apparatus is 1-2 milliseconds but some new devices have been Drive developed that have dead times of 0.3-0.6 ms Single Mixing Stopped-Flow
10.7.3 Stopped-Flow Measurements in real time not requiring large amounts of reagents (as little as 1 cm3 may be sufficient) the time resolution is a little faster then for continuous flow. Fixed probe strategy. The usual dead time of a stopped flow apparatus is 1–2 milliseconds but some new devices have been developed that have dead times of 0.3–0.6 ms 10.7 Reaction rate and rate equation